This chemical is widely used in bioconjugation and labeling applications due to its unique functional groups. The diazo group allows for efficient and selective coupling with aromatic compounds, making it valuable in protein and antibody labeling. The biotin moiety enables strong binding to avidin or streptavidin, facilitating detection, purification, and immobilization in assays like ELISA, Western blotting, and pull-down experiments. The PEG3 spacer enhances solubility and reduces steric hindrance, improving the efficiency of binding interactions. Additionally, the azide group enables click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), for attaching biomolecules or probes. This makes it a versatile tool in chemical biology, drug discovery, and materials science for creating complex molecular architectures.