Used in bioconjugation and chemical biology research, this compound facilitates the labeling and detection of biomolecules. It is particularly valuable in click chemistry reactions, where the azide group reacts with alkynes to form stable triazole linkages. The biotin moiety allows for strong binding to streptavidin or avidin, enabling purification or visualization of tagged molecules. The PEG4 spacer enhances solubility and reduces steric hindrance, improving reaction efficiency. Commonly applied in proteomics, nucleic acid studies, and cellular imaging to track and analyze molecular interactions. Its orthogonal protecting group, Dde, ensures selective deprotection in multi-step synthesis processes.