This compound is widely utilized in bioconjugation and biochemical research due to its unique properties. It serves as a linker molecule, enabling the attachment of biotin to various biomolecules, such as proteins, peptides, or nucleic acids. The PEG3 spacer enhances solubility and reduces steric hindrance, improving binding efficiency in applications like affinity purification, immunoassays, and diagnostic assays. Its amine group allows for further functionalization, making it a versatile tool in drug delivery systems, targeted therapies, and the development of biosensors. Additionally, it is commonly used in streptavidin-biotin binding studies, leveraging the strong interaction for precise detection and analysis in molecular biology and biotechnology research.