N-Biotin-n-bis(peg4-acid) is widely used in bioconjugation and biochemical research due to its unique properties. Its biotin moiety allows for strong and specific binding to avidin or streptavidin, making it valuable in applications like affinity purification, immunoassays, and diagnostic tests. The PEG4 spacer enhances solubility and reduces steric hindrance, facilitating efficient binding interactions. This compound is also employed in the development of drug delivery systems, where biotinylation of therapeutic agents or nanoparticles enables targeted delivery to specific cells or tissues. Additionally, it plays a role in labeling and tracking biomolecules in cellular imaging and molecular biology studies, providing a versatile tool for researchers.