This compound is widely used in bioconjugation and click chemistry applications, particularly in the fields of biochemistry and molecular biology. It serves as a versatile linker for attaching biotin to various biomolecules, enabling the use of streptavidin-based detection systems. The PEG4 spacer enhances solubility and reduces steric hindrance, improving the efficiency of binding interactions. The azide group allows for click chemistry reactions, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC), facilitating the labeling of proteins, peptides, or other molecules. Additionally, the Dde protecting group can be selectively removed under mild conditions, enabling controlled and site-specific biotinylation. This makes it a valuable tool for studying protein interactions, developing diagnostic assays, and designing targeted drug delivery systems.