Used extensively in bioconjugation techniques, this compound serves as a crucial linker for attaching biotin to proteins, peptides, or other biomolecules. Its PEG4 spacer enhances solubility and reduces steric hindrance, making it ideal for applications in immunoassays, affinity purification, and targeted drug delivery systems. The NHS carbonate group enables efficient and stable carbamate bond formation with primary amines, facilitating the biotinylation of various substrates. This enables the use of streptavidin-biotin interactions in diagnostic assays, fluorescence microscopy, and molecular biology research, providing high specificity and sensitivity in detecting or isolating target molecules.