N-(3-Azidopropyl)biotinamide is widely used in bioconjugation and labeling applications due to its unique combination of biotin and azide functional groups. The biotin moiety allows for strong binding to avidin or streptavidin, enabling its use in affinity purification, detection, and immobilization of biomolecules. The azide group facilitates click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), making it valuable for tagging and crosslinking biomolecules in proteomics, cellular imaging, and drug delivery systems. It is also employed in the development of biotinylated probes for studying protein-protein interactions and post-translational modifications. Additionally, its dual functionality makes it a versatile tool in the synthesis of biotinylated polymers and surfaces for biosensor applications.