This chemical is widely used in bioconjugation processes, particularly for labeling proteins, antibodies, and other biomolecules with biotin. Its application is crucial in techniques such as ELISA, Western blotting, and immunohistochemistry, where biotinylation enhances detection sensitivity. The compound facilitates the attachment of biotin to primary amines on target molecules, enabling subsequent binding to streptavidin or avidin for visualization or purification. It is also employed in affinity chromatography to isolate specific biomolecules. Additionally, it plays a role in studying protein-protein interactions and cellular processes by tagging proteins for tracking and analysis. Its stability and efficiency make it a preferred choice in biochemical and biomedical research.