This compound is widely used in bioconjugation and click chemistry applications. It serves as a linker molecule, enabling the attachment of biotin to various biomolecules or surfaces through azide-alkyne cycloaddition reactions. Its PEG spacer enhances solubility and reduces steric hindrance, making it ideal for labeling proteins, peptides, or other biological targets. In research, it is commonly employed for creating biotinylated probes for affinity purification, immunodetection, or imaging studies. Additionally, it finds utility in drug delivery systems, where biotin acts as a targeting ligand for receptors overexpressed in certain diseases. Its versatility makes it a valuable tool in biochemistry, molecular biology, and nanotechnology.