N-Succinimidyl 6-Biotinamidohexanoate is widely used in biochemical and biotechnological research for biotinylation of proteins, antibodies, and other biomolecules. It serves as a key reagent in labeling studies, enabling the attachment of biotin to target molecules through primary amines. This biotinylation facilitates the use of streptavidin or avidin-based detection systems, which are essential in techniques like ELISA, Western blotting, and immunofluorescence. Its long spacer arm enhances accessibility, reducing steric hindrance and improving binding efficiency. Additionally, it is employed in affinity purification, where biotinylated molecules are isolated using streptavidin-coated beads. The compound is also utilized in the development of diagnostic assays and in studying protein-protein interactions, making it a versatile tool in molecular biology and diagnostics.