This chemical is widely used in bioconjugation processes, particularly for labeling proteins, peptides, and other biomolecules with biotin. Its primary application lies in facilitating the attachment of biotin to target molecules, enabling their detection or purification through biotin-streptavidin interactions. This is especially useful in techniques like ELISA, Western blotting, and immunohistochemistry, where biotinylated molecules are detected using streptavidin-linked enzymes or fluorescent tags. Additionally, it is employed in affinity chromatography to isolate specific biomolecules by leveraging the strong binding affinity between biotin and streptavidin. The compound’s water-soluble nature and efficient reactivity make it a valuable tool in molecular biology and biochemical research.