This compound is widely used in bioconjugation and biochemical research due to its unique properties. The biotin moiety allows for strong binding to avidin or streptavidin, making it valuable in applications like protein labeling, detection, and purification. The PEG3 spacer enhances solubility and reduces steric hindrance, improving accessibility in binding assays. The thiol (-SH) group enables covalent attachment to maleimide or gold surfaces, facilitating the functionalization of nanoparticles, biosensors, and other surfaces. It is particularly useful in constructing biotinylated probes for studying protein interactions, developing diagnostic assays, and designing targeted drug delivery systems.