This compound is widely used in bioconjugation and labeling applications due to its unique functional groups. The biotin moiety allows for strong and specific binding to avidin or streptavidin, making it valuable in affinity purification, immunoassays, and diagnostic assays. The PEG4 spacer enhances solubility and reduces steric hindrance, improving the efficiency of binding interactions. The amide group provides stability, while the C6 linker offers flexibility. The azide group enables click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), facilitating the attachment of biomolecules, probes, or other functional groups in research and drug development. It is commonly employed in the study of protein-protein interactions, cell surface labeling, and the development of targeted therapeutics.