This compound is widely used in bioconjugation processes, particularly for labeling biomolecules such as proteins, peptides, and antibodies. Its NHS ester group reacts efficiently with primary amines, enabling stable amide bond formation, while the PEG spacer enhances solubility and reduces steric hindrance. This makes it valuable in applications like drug delivery systems, where it helps attach targeting ligands to therapeutic agents. It is also utilized in diagnostic assays, where it facilitates the labeling of biomolecules for detection and imaging purposes. Additionally, its biotin moiety allows for strong binding to streptavidin or avidin, making it useful in affinity chromatography and pull-down assays for protein isolation and analysis.