This compound is widely utilized in bioconjugation and labeling applications due to its dual functional groups. The azide group enables click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), making it suitable for attaching biomolecules or probes in biological research. The biotin moiety allows for strong and specific binding to avidin or streptavidin, facilitating purification, detection, or immobilization of target molecules in assays like ELISA, Western blotting, or affinity chromatography. The PEG spacers enhance solubility and reduce steric hindrance, ensuring efficient interactions in complex biological systems. This compound is particularly valuable in drug delivery, molecular imaging, and the development of diagnostic tools.