Ibrutinib-biotin is primarily used in biochemical research and drug development studies. It serves as a valuable tool for investigating the mechanism of action of ibrutinib, a Bruton's tyrosine kinase (BTK) inhibitor. By conjugating biotin to ibrutinib, researchers can leverage biotin's strong affinity for avidin or streptavidin to isolate, detect, or purify BTK and related proteins in cellular assays. This compound is particularly useful in pull-down assays, Western blotting, and fluorescence microscopy to study protein interactions, signaling pathways, and drug-target engagement. Additionally, it aids in the development of targeted therapies for conditions like chronic lymphocytic leukemia and other B-cell malignancies by providing insights into drug efficacy and resistance mechanisms.