N-GlcNAc-Biotin is widely used in glycobiology research for studying protein glycosylation. It serves as a metabolic labeling tool, allowing scientists to track and analyze glycosylated proteins in cells. This compound is particularly valuable in identifying O-GlcNAc-modified proteins, which play critical roles in cellular signaling, metabolism, and disease processes. By incorporating biotin, it enables efficient isolation and detection of glycosylated proteins using streptavidin-based purification techniques. Additionally, it aids in visualizing glycosylation patterns in live cells or tissues, providing insights into the dynamic regulation of protein modifications. Its applications extend to investigating diseases like cancer, diabetes, and neurodegenerative disorders, where aberrant glycosylation is often observed.