N-Biotinyl Glycine is primarily utilized in biochemical research and bioconjugation processes. It serves as a versatile linker molecule for attaching biotin to proteins, peptides, or other biomolecules, enabling their detection, purification, or immobilization in various assays. This compound is particularly valuable in techniques like ELISA, Western blotting, and affinity chromatography, where biotin-streptavidin interactions are exploited for high-specificity binding. Additionally, it is used in the development of biotinylated probes for studying molecular interactions, cellular localization, and signal transduction pathways. Its application extends to drug delivery systems, where it aids in targeted delivery mechanisms by leveraging biotin receptors on specific cell types.