Diketone-PEG4-Biotin is widely used in bioconjugation and biochemical research due to its unique properties. It serves as a versatile linker in the development of biotinylated probes and reagents, enabling the study of protein interactions and cellular processes. The diketone group allows for specific covalent binding to hydrazide or aminooxy-functionalized molecules, facilitating the attachment of biotin to various biomolecules. This compound is particularly valuable in affinity purification, where biotin’s strong interaction with streptavidin or avidin is utilized to isolate and analyze target proteins or nucleic acids.

Additionally, it is employed in fluorescence labeling and imaging techniques to track biomolecules in live cells or tissues, as well as in specific assays such as ELISA, Western Blot, and flow cytometry. Its PEG4 spacer enhances solubility and reduces steric hindrance, improving the efficiency of binding and detection. This makes it a critical tool in proteomics, drug discovery, diagnostic applications, targeted drug delivery, and nanotechnology for surface coating of nanoparticles to improve targeting in disease diagnosis and therapy.