This compound is primarily utilized in bioconjugation and protein modification processes. Its structure, featuring an activated ester group and a biotin moiety, makes it highly effective for attaching biotin to proteins, peptides, or other biomolecules. The biotinylation enables the use of streptavidin-biotin interactions, which are widely employed in assays like ELISA, Western blotting, and immunofluorescence for detection and purification purposes. Additionally, it is used in the development of diagnostic tools and research applications where precise labeling and targeting of biomolecules are required. The polyethylene glycol (PEG) linker in the compound enhances solubility and reduces steric hindrance, improving the efficiency of bioconjugation reactions.