This compound is widely used in bioconjugation and biochemical research. It serves as a versatile linker for attaching biotin to proteins, peptides, or other biomolecules. The iodoacetamide group reacts specifically with cysteine residues, enabling precise labeling of thiol-containing molecules. The PEG2 spacer enhances solubility and reduces steric hindrance, improving binding efficiency. Biotinylation allows for the detection, purification, or immobilization of target molecules using streptavidin-based systems, such as ELISA, flow cytometry, or affinity chromatography. It is particularly valuable in studying protein interactions, cellular processes, and developing diagnostic assays.