Biotin-PEG7-thiourea is widely used in biochemical research and pharmaceutical applications due to its unique properties. It is primarily employed as a linker molecule in bioconjugation techniques, facilitating the attachment of biotin to various biomolecules such as proteins, peptides, or nucleic acids. This enables the use of biotin-streptavidin interactions for detection, purification, or immobilization purposes in assays like ELISA, Western blotting, or affinity chromatography. The PEG7 spacer in the molecule enhances solubility and reduces steric hindrance, making it ideal for applications requiring flexibility and accessibility. Additionally, the thiourea group provides a stable covalent bond when reacting with amine-containing compounds, ensuring robust conjugation. This chemical is also utilized in targeted drug delivery systems, where biotinylated ligands are used to direct therapeutic agents to specific cells or tissues, leveraging the high affinity of biotin for streptavidin or avidin. Furthermore, it finds use in the development of diagnostic probes and imaging agents, where biotinylation aids in signal amplification and detection sensitivity.