Biotin-PEG8-amine is widely used in bioconjugation and labeling applications due to its ability to link biotin with various biomolecules. It serves as a versatile reagent in the preparation of biotinylated probes, which are essential for techniques like ELISA, immunohistochemistry, and flow cytometry. The PEG8 spacer enhances solubility and reduces steric hindrance, improving the efficiency of binding to streptavidin or avidin. This compound is also employed in the development of drug delivery systems, where it facilitates targeted delivery by conjugating therapeutic agents to biotin-binding proteins. Additionally, it plays a role in surface modification of nanoparticles and biomaterials, enabling specific interactions in diagnostic and therapeutic applications.