Biotin hydrazide is widely used in biochemical research for labeling and detecting biomolecules. It is particularly effective in bioconjugation techniques, where it attaches to aldehydes and ketones, enabling the tagging of carbohydrates, glycoproteins, and other biomolecules with biotin. This biotinylation allows for the use of streptavidin or avidin-based detection systems, enhancing the sensitivity and specificity of assays like ELISA, Western blotting, and immunohistochemistry. Additionally, it plays a role in studying protein interactions and cellular processes by facilitating the tracking and purification of target molecules. Its applications extend to drug delivery systems, where it helps in targeting specific cells or tissues.