This compound is primarily used in advanced biochemical research, particularly in the study of protein interactions and bioconjugation processes. Its structure, featuring a biotin moiety, makes it highly suitable for applications in affinity chromatography and protein labeling. The compound is often employed to tag or immobilize proteins, enabling their purification or detection in complex biological samples. Additionally, its deuterated form allows for use in mass spectrometry and NMR studies, where isotopic labeling is crucial for tracking molecular behavior and interactions. It is also utilized in the development of biosensors and diagnostic assays, leveraging its strong binding affinity to avidin or streptavidin for signal amplification and specificity.