Biotin solution is widely used in molecular biology and biochemistry for various applications. It is commonly employed as a labeling agent for proteins, nucleic acids, and other biomolecules due to its strong affinity for avidin and streptavidin. This property makes it essential in techniques like ELISA, Western blotting, and immunohistochemistry, where it helps in the detection and visualization of target molecules. In cell culture studies, biotin solution is added to media to support cell growth and metabolism, particularly in cases where biotin deficiency may affect cellular functions. Additionally, it is used in diagnostic assays to measure biotin levels in clinical samples, aiding in the diagnosis of biotin-related disorders. Its role in enhancing the sensitivity and specificity of detection methods makes it a valuable tool in research and diagnostics.