Blotto in TBS is widely used as a blocking agent in immunoblotting and other immunoassay techniques. It helps prevent non-specific binding of antibodies to the membrane, ensuring more accurate and reliable results. Typically, it is applied after transferring proteins to the membrane and before incubating with primary antibodies. By blocking potential binding sites, it reduces background noise and enhances the specificity of the detection process. This makes it a crucial component in protein analysis, particularly in Western blotting, where clear and precise identification of target proteins is essential. Additionally, it is compatible with various detection systems, including chemiluminescence and fluorescence, making it versatile for different experimental setups.