Casein-Tween-20 in TBS is widely used in immunoassays and protein research as a blocking agent to prevent non-specific binding. It is particularly effective in ELISA (Enzyme-Linked Immunosorbent Assay) and Western blotting techniques, where it coats the surface of membranes or microplates to minimize background noise and improve the specificity of antibody-antigen interactions. The combination of casein and Tween-20 in Tris-buffered saline (TBS) creates a stable and efficient blocking solution, ensuring accurate and reliable results in diagnostic and research applications. Additionally, it is compatible with various detection systems, making it a versatile choice for laboratory workflows.