BSA Blocking Buffer in TBS with Tween-20 is widely used in immunoassays and molecular biology techniques to reduce non-specific binding. It is commonly applied in Western blotting, ELISA, and immunohistochemistry to block unoccupied binding sites on membranes or microplates, ensuring that antibodies bind only to their specific targets. The inclusion of Tween-20 helps minimize background noise by preventing non-specific interactions. This buffer enhances the accuracy and reliability of experimental results, making it a critical component in protein detection and analysis workflows.