Gelatin blocking buffer in TBS is widely used in immunoassays and immunohistochemistry to reduce non-specific binding of antibodies. It is particularly effective in blocking hydrophobic surfaces on membranes or tissue sections, ensuring that antibodies bind only to their specific targets. This buffer is commonly applied during Western blotting, ELISA, and other protein detection techniques to improve signal-to-noise ratios. Its gelatin component provides a stable and efficient blocking medium, making it a preferred choice for researchers aiming to achieve clear and accurate results in protein analysis. Additionally, it is compatible with various detection systems and maintains protein integrity during the blocking process.