FCS Blocking Buffer in PBS is commonly used in immunological and biochemical assays to reduce non-specific binding. It is particularly effective in techniques such as ELISA, Western blotting, and immunofluorescence, where it helps block unoccupied binding sites on surfaces, thereby minimizing background noise and improving signal clarity. The buffer is also utilized in cell-based assays to prevent non-specific interactions between antibodies and cells, ensuring more accurate and reliable results. Its compatibility with phosphate-buffered saline (PBS) makes it suitable for a wide range of biological applications, enhancing the specificity and sensitivity of detection methods.