FCS Blocking Buffer in TBS is widely used in immunoassays and molecular biology techniques to reduce non-specific binding. It is particularly effective in ELISA, Western blotting, and immunohistochemistry, where it blocks unoccupied binding sites on membranes or microplates, ensuring accurate and specific detection of target proteins or antibodies. The inclusion of fetal calf serum (FCS) provides proteins that competitively inhibit non-specific interactions, improving signal-to-noise ratios. This buffer is also compatible with Tris-buffered saline (TBS), making it suitable for a variety of experimental conditions. Its use enhances the reliability and reproducibility of results in protein and antibody-based assays.