3-Hydroxyphenylboronic acid is widely used in organic synthesis, particularly as a key reagent in Suzuki-Miyaura cross-coupling reactions. These reactions are essential for forming carbon-carbon bonds, making it valuable in the production of pharmaceuticals, agrochemicals, and advanced materials. Its boronic acid group enables efficient coupling with aryl halides, facilitating the creation of complex organic molecules.

In the field of biochemistry, this compound is employed in the development of sensors and probes. It exhibits strong affinity for diols, making it useful in detecting sugars and other biologically relevant molecules. This property is leveraged in glucose monitoring systems and other diagnostic tools.

Additionally, 3-Hydroxyphenylboronic acid is utilized in polymer chemistry, where it serves as a monomer or cross-linking agent. It contributes to the development of stimuli-responsive materials, such as hydrogels, which can change properties in response to pH or specific analytes. This makes it suitable for applications in drug delivery systems and smart materials.