2-Isopropylmalic acid is primarily used in biochemical research as an intermediate in the biosynthesis of leucine, an essential amino acid. It plays a significant role in studying metabolic pathways, particularly in understanding the mechanisms of enzyme-catalyzed reactions in the leucine biosynthesis process. Additionally, it is utilized in the development of microbial fermentation processes for the production of amino acids and other bioactive compounds. Its application extends to the field of enzymology, where it serves as a substrate for investigating the activity and specificity of enzymes like isopropylmalate dehydrogenase. This compound is also relevant in genetic engineering and metabolic engineering studies aimed at optimizing amino acid production in microorganisms.