β-NF-JQ1 is primarily used in research settings to study its effects on bromodomain proteins, particularly BRD4. It is a valuable tool in understanding the role of these proteins in gene regulation and their involvement in diseases such as cancer. Researchers utilize β-NF-JQ1 to explore potential therapeutic strategies by targeting epigenetic mechanisms. Its application extends to investigating inflammatory responses and immune system modulation, providing insights into conditions like autoimmune disorders. Additionally, it aids in the development of novel treatments by serving as a reference compound for designing more effective inhibitors.