BET-IN-2 is primarily utilized in biochemical research, particularly in the study of protein-protein interactions and epigenetic regulation. It serves as a potent inhibitor of bromodomain and extraterminal (BET) proteins, which play a crucial role in controlling gene expression. By inhibiting these proteins, BET-IN-2 helps researchers investigate the mechanisms underlying various diseases, including cancer, inflammation, and cardiovascular disorders. Its application extends to drug discovery, where it is used to identify potential therapeutic targets and develop novel treatments for conditions linked to aberrant gene expression. Additionally, BET-IN-2 is employed in cellular assays to understand the functional roles of BET proteins in chromatin remodeling and transcriptional regulation.