I-BET762 carboxylic acid is primarily utilized in the field of medicinal chemistry and drug development. It serves as a key intermediate in the synthesis of BET bromodomain inhibitors, which are important for targeting epigenetic regulation. These inhibitors play a significant role in research focused on cancer therapy, inflammation, and other diseases influenced by gene expression. The compound is particularly valuable in the development of small-molecule drugs that modulate protein-protein interactions, aiding in the discovery of novel therapeutic agents. Its application extends to biochemical assays and preclinical studies, where it helps in understanding the mechanisms of bromodomain-containing proteins and their impact on disease pathways.