Used in microbiology for the cultivation and isolation of fastidious microorganisms, particularly anaerobic bacteria. Supports the growth of organisms that require complex nutrients and reduced oxidation-reduction potential. Commonly applied in clinical laboratories for diagnosing infections involving anaerobes, such as those from gastrointestinal, dental, or wound sources. Often supplemented with blood or other growth factors to enhance recovery of sensitive strains. Also used in research settings to study microbial metabolism and interactions under controlled anaerobic conditions.