PIPES sesquisodium salt is widely used as a buffering agent in biochemical and molecular biology research. It is particularly effective in maintaining stable pH levels in cell culture media, enzyme assays, and protein purification processes. Its buffering range, typically between pH 6.1 and 7.5, makes it suitable for experiments involving biological systems that require near-neutral conditions. Additionally, it is often employed in electrophoresis and chromatography techniques due to its minimal interference with biological molecules and low reactivity with metal ions. PIPES sesquisodium salt is also utilized in studies involving cell physiology and microbiology, where precise pH control is critical for maintaining cell viability and function.