Used as a buffering agent in biochemical and molecular biology applications, particularly in systems where a stable pH range between 8.6 and 10.0 is required. Its zwitterionic nature helps maintain pH stability without interfering with enzymatic reactions. Commonly found in cell lysis buffers, protein purification processes, and electrophoresis techniques at higher pH values. Also utilized in some diagnostic assays and pharmaceutical formulations due to its low toxicity and good solubility in water.