2X Saline Sodium Citrate (SSC) Buffer is widely used in molecular biology and biochemistry for nucleic acid hybridization and washing procedures. It is essential in Southern, Northern, dot, slot blotting, microarray, and in situ hybridization techniques, where it controls hybridization stringency through salt concentration during post-hybridization washes. The buffer provides a stable pH (typically 7.0) and optimal ionic strength for stable binding of DNA/RNA probes to target nucleic acids, preventing non-specific interactions. Its formulation supports various research and diagnostic applications in nucleic acid detection and analysis.