POPSO buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.2 to 8.5. It is particularly suitable for applications involving enzyme assays, cell culture media, and protein purification processes where maintaining a stable pH is critical. POPSO buffer is also utilized in electrophoresis techniques, such as SDS-PAGE, to ensure consistent pH conditions during protein separation. Additionally, it finds application in nucleic acid hybridization and other sensitive biochemical reactions where precise pH control is essential for optimal results. Its low metal ion contamination makes it a preferred choice in studies involving metal-sensitive enzymes or proteins.