Phosphate Buffered Saline without Potassium is widely used in biological and medical research for maintaining a stable pH environment in cell cultures and experiments. It is particularly useful in washing cells during preparation for assays, as it helps remove residual media or contaminants without disrupting cellular integrity. This solution is also employed in immunohistochemistry and immunofluorescence techniques to rinse tissue samples, ensuring optimal antibody binding and reducing background noise. Additionally, it serves as a diluent for reagents and a medium for transporting or storing biological samples, as it closely mimics the osmotic balance of bodily fluids. Its lack of potassium makes it suitable for applications where potassium interference must be avoided, such as certain enzymatic assays or studies involving potassium-sensitive cells.