Glycine buffer in PBS is widely used in biological and biochemical research due to its ability to maintain a stable pH environment. It is commonly employed in protein and antibody purification processes, particularly in affinity chromatography, where it helps elute proteins from columns without causing denaturation. This buffer is also utilized in electrophoresis techniques, such as SDS-PAGE, to ensure optimal separation of proteins. Additionally, it plays a critical role in cell culture applications, providing a balanced ionic environment for cell growth and maintenance. In immunoassays, glycine buffer in PBS is often used for blocking and washing steps to minimize non-specific binding, enhancing the accuracy of results. Its compatibility with various biological systems makes it a versatile choice in laboratory workflows.