Mcilvaine Buffer is widely used in various biochemical and microbiological applications due to its ability to maintain a stable pH. It is commonly employed in enzyme assays, where precise pH control is crucial for accurate results. The buffer is also utilized in cell culture studies to provide a consistent environment for cell growth and metabolism. In molecular biology, Mcilvaine Buffer is often used in nucleic acid extraction and purification processes, ensuring optimal conditions for enzymatic reactions. Additionally, it finds application in chromatography, particularly in ion-exchange and affinity chromatography, to maintain the desired pH during separation processes. Its versatility and reliability make it a valuable tool in research and diagnostic laboratories.