Alkaline Phosphatase Buffer (0.5M, pH 9.0, 10×) is widely used in molecular biology and biochemistry laboratories for various enzymatic reactions. It is particularly essential in dephosphorylation processes, where it provides the optimal pH environment for alkaline phosphatase enzymes to function efficiently. This buffer is commonly employed in DNA and RNA manipulation, such as removing phosphate groups from the 5' ends of nucleic acids to prevent self-ligation during cloning procedures. Additionally, it is utilized in immunoassays, including ELISA, to ensure proper enzyme-substrate interactions for accurate detection and quantification of target molecules. Its stable pH and concentration make it a reliable choice for maintaining consistent reaction conditions in high-throughput applications.