Used primarily in biochemical and molecular biology laboratories to maintain stable pH levels in various experiments. It is commonly employed in DNA and RNA extraction processes to ensure optimal conditions for enzymatic reactions. The buffer is also utilized in cell culture media to support cell growth and maintain physiological pH. In diagnostic assays, it helps in stabilizing reagents and samples, ensuring accurate and reproducible results. Additionally, it is applied in chromatography techniques to improve separation efficiency and maintain consistent pH during analysis. Its buffering capacity makes it suitable for use in pharmaceutical formulations to control the pH of injectable drugs and oral medications.