Phosphate Buffer (PB Buffer) is widely used in biological and chemical research due to its ability to maintain a stable pH environment. It is commonly employed in cell culture applications to provide optimal conditions for cell growth and maintenance. In molecular biology, PB Buffer is essential for procedures such as DNA and RNA extraction, purification, and electrophoresis, ensuring the integrity of nucleic acids during processing. It is also utilized in immunohistochemistry and immunofluorescence techniques to prepare tissue samples and maintain antibody activity. Additionally, PB Buffer plays a critical role in enzyme assays, where consistent pH is necessary for accurate enzyme activity measurement. Its compatibility with various biochemical reactions makes it a versatile tool in laboratory settings.