Phosphate Buffer (PB Buffer) is widely used in biological and chemical research due to its ability to maintain a stable pH. It is commonly employed in cell culture applications to provide a consistent environment for cell growth and viability. In molecular biology, PB Buffer is utilized for washing and resuspending cells during experiments such as immunofluorescence and flow cytometry. It also serves as a key component in protein purification processes, ensuring proteins remain stable and functional. Additionally, PB Buffer is used in diagnostic assays, including ELISA, to maintain optimal conditions for antibody-antigen interactions. Its buffering capacity makes it suitable for use in electrophoresis and chromatography techniques, where pH stability is critical for accurate results.