Phosphate Buffer (PB Buffer) is widely used in biological and chemical research for maintaining stable pH levels. It is essential in cell culture experiments to provide a consistent environment for cell growth and viability. In molecular biology, PB Buffer is commonly employed in techniques such as DNA and RNA extraction, electrophoresis, and protein purification, ensuring optimal conditions for enzymatic reactions. It is also utilized in immunohistochemistry and immunofluorescence for tissue sample preparation and antibody dilutions. Additionally, PB Buffer plays a critical role in diagnostic assays, including ELISA, by maintaining the pH required for accurate antigen-antibody interactions. Its buffering capacity makes it suitable for use in various laboratory applications, including chromatography and spectroscopy.